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osteogenic induction reagents  (Valiant Co Ltd)

 
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    Structured Review

    Valiant Co Ltd osteogenic induction reagents
    Characterization and differentiation potential of hPDLCs. (A) Schematic representation of hPDLCs mechanical memory, including the experimental grouping of cells cultured on substrates with soft (So) and stiff (St) matrix stiffness to evaluate mechanical memory effects. (B) Flow cytometry identification of hPDLCs using cell surface markers (presence of mesenchymal markers CD90 and CD146 and absence of hematopoietic markers CD34 and CD45). (C) The representative images of hPDLCs lipogenic ability. (D) The representative images of hPDLCs <t>osteogenic</t> ability. Scale bars in (c) = 50 μm, in (d) = 200 μm.
    Osteogenic Induction Reagents, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/osteogenic induction reagents/product/Valiant Co Ltd
    Average 95 stars, based on 240 article reviews
    osteogenic induction reagents - by Bioz Stars, 2026-03
    95/100 stars

    Images

    1) Product Images from "Matrix Stiffness-Induced Mechanical Memory of Periodontal Ligament Cell Promotes Osteogenesis in Periodontal Bone Repair"

    Article Title: Matrix Stiffness-Induced Mechanical Memory of Periodontal Ligament Cell Promotes Osteogenesis in Periodontal Bone Repair

    Journal: International Dental Journal

    doi: 10.1016/j.identj.2025.103992

    Characterization and differentiation potential of hPDLCs. (A) Schematic representation of hPDLCs mechanical memory, including the experimental grouping of cells cultured on substrates with soft (So) and stiff (St) matrix stiffness to evaluate mechanical memory effects. (B) Flow cytometry identification of hPDLCs using cell surface markers (presence of mesenchymal markers CD90 and CD146 and absence of hematopoietic markers CD34 and CD45). (C) The representative images of hPDLCs lipogenic ability. (D) The representative images of hPDLCs osteogenic ability. Scale bars in (c) = 50 μm, in (d) = 200 μm.
    Figure Legend Snippet: Characterization and differentiation potential of hPDLCs. (A) Schematic representation of hPDLCs mechanical memory, including the experimental grouping of cells cultured on substrates with soft (So) and stiff (St) matrix stiffness to evaluate mechanical memory effects. (B) Flow cytometry identification of hPDLCs using cell surface markers (presence of mesenchymal markers CD90 and CD146 and absence of hematopoietic markers CD34 and CD45). (C) The representative images of hPDLCs lipogenic ability. (D) The representative images of hPDLCs osteogenic ability. Scale bars in (c) = 50 μm, in (d) = 200 μm.

    Techniques Used: Cell Culture, Flow Cytometry

    Primed hPDLCs exhibit different osteogenic abilities on substrates with varying stiffness. (A) Representative images of ALP staining at days 7 and 14. (B) Quantification of ALP staining area using ImageJ software. (n = 3, * P < . 05; ** P < . 01; *** P < . 001; **** P < . 0001, ns, no significance, data are described as the mean ± SD, comparisons were analyzed by two-way ANOVA adjusted by the Tukey test). (C-F) mRNA expression levels of osteogenic markers: (C) ALP, (d) COL-1, (E) OPN, and (F) RUNX2 at days 4, 7, and 14. (n = 3, * P < . 05; ** P < . 01; *** P < . 001; **** P < . 0001, ns, no significance, data are described as the mean ± SD, comparisons were analyzed by two-way ANOVA adjusted by the Tukey test). Scale bars in (a) = 200 μm.
    Figure Legend Snippet: Primed hPDLCs exhibit different osteogenic abilities on substrates with varying stiffness. (A) Representative images of ALP staining at days 7 and 14. (B) Quantification of ALP staining area using ImageJ software. (n = 3, * P < . 05; ** P < . 01; *** P < . 001; **** P < . 0001, ns, no significance, data are described as the mean ± SD, comparisons were analyzed by two-way ANOVA adjusted by the Tukey test). (C-F) mRNA expression levels of osteogenic markers: (C) ALP, (d) COL-1, (E) OPN, and (F) RUNX2 at days 4, 7, and 14. (n = 3, * P < . 05; ** P < . 01; *** P < . 001; **** P < . 0001, ns, no significance, data are described as the mean ± SD, comparisons were analyzed by two-way ANOVA adjusted by the Tukey test). Scale bars in (a) = 200 μm.

    Techniques Used: Staining, Software, Expressing



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    Characterization and differentiation potential of hPDLCs. (A) Schematic representation of hPDLCs mechanical memory, including the experimental grouping of cells cultured on substrates with soft (So) and stiff (St) matrix stiffness to evaluate mechanical memory effects. (B) Flow cytometry identification of hPDLCs using cell surface markers (presence of mesenchymal markers CD90 and CD146 and absence of hematopoietic markers CD34 and CD45). (C) The representative images of hPDLCs lipogenic ability. (D) The representative images of hPDLCs <t>osteogenic</t> ability. Scale bars in (c) = 50 μm, in (d) = 200 μm.
    Osteogenic Induction Reagents, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/osteogenic induction reagents/product/Valiant Co Ltd
    Average 95 stars, based on 1 article reviews
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    Characterization and differentiation potential of hPDLCs. (A) Schematic representation of hPDLCs mechanical memory, including the experimental grouping of cells cultured on substrates with soft (So) and stiff (St) matrix stiffness to evaluate mechanical memory effects. (B) Flow cytometry identification of hPDLCs using cell surface markers (presence of mesenchymal markers CD90 and CD146 and absence of hematopoietic markers CD34 and CD45). (C) The representative images of hPDLCs lipogenic ability. (D) The representative images of hPDLCs <t>osteogenic</t> ability. Scale bars in (c) = 50 μm, in (d) = 200 μm.
    Bmscs Induction Differentiation Reagent Kit (Osteogenic, Adipogenic And Chondrogenic), supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Characterization and differentiation potential of hPDLCs. (A) Schematic representation of hPDLCs mechanical memory, including the experimental grouping of cells cultured on substrates with soft (So) and stiff (St) matrix stiffness to evaluate mechanical memory effects. (B) Flow cytometry identification of hPDLCs using cell surface markers (presence of mesenchymal markers CD90 and CD146 and absence of hematopoietic markers CD34 and CD45). (C) The representative images of hPDLCs lipogenic ability. (D) The representative images of hPDLCs osteogenic ability. Scale bars in (c) = 50 μm, in (d) = 200 μm.

    Journal: International Dental Journal

    Article Title: Matrix Stiffness-Induced Mechanical Memory of Periodontal Ligament Cell Promotes Osteogenesis in Periodontal Bone Repair

    doi: 10.1016/j.identj.2025.103992

    Figure Lengend Snippet: Characterization and differentiation potential of hPDLCs. (A) Schematic representation of hPDLCs mechanical memory, including the experimental grouping of cells cultured on substrates with soft (So) and stiff (St) matrix stiffness to evaluate mechanical memory effects. (B) Flow cytometry identification of hPDLCs using cell surface markers (presence of mesenchymal markers CD90 and CD146 and absence of hematopoietic markers CD34 and CD45). (C) The representative images of hPDLCs lipogenic ability. (D) The representative images of hPDLCs osteogenic ability. Scale bars in (c) = 50 μm, in (d) = 200 μm.

    Article Snippet: Analogously, osteogenic differentiation was induced in 10% FBS α-MEM medium supplemented with osteogenic induction reagents (consisting of 0.1 μM/L dexamethasone, 10 mM/L β-glycerophosphate, and 50 μg/mL ascorbic acid; MP Biomedicals and Sigma, US) for 21 days.

    Techniques: Cell Culture, Flow Cytometry

    Primed hPDLCs exhibit different osteogenic abilities on substrates with varying stiffness. (A) Representative images of ALP staining at days 7 and 14. (B) Quantification of ALP staining area using ImageJ software. (n = 3, * P < . 05; ** P < . 01; *** P < . 001; **** P < . 0001, ns, no significance, data are described as the mean ± SD, comparisons were analyzed by two-way ANOVA adjusted by the Tukey test). (C-F) mRNA expression levels of osteogenic markers: (C) ALP, (d) COL-1, (E) OPN, and (F) RUNX2 at days 4, 7, and 14. (n = 3, * P < . 05; ** P < . 01; *** P < . 001; **** P < . 0001, ns, no significance, data are described as the mean ± SD, comparisons were analyzed by two-way ANOVA adjusted by the Tukey test). Scale bars in (a) = 200 μm.

    Journal: International Dental Journal

    Article Title: Matrix Stiffness-Induced Mechanical Memory of Periodontal Ligament Cell Promotes Osteogenesis in Periodontal Bone Repair

    doi: 10.1016/j.identj.2025.103992

    Figure Lengend Snippet: Primed hPDLCs exhibit different osteogenic abilities on substrates with varying stiffness. (A) Representative images of ALP staining at days 7 and 14. (B) Quantification of ALP staining area using ImageJ software. (n = 3, * P < . 05; ** P < . 01; *** P < . 001; **** P < . 0001, ns, no significance, data are described as the mean ± SD, comparisons were analyzed by two-way ANOVA adjusted by the Tukey test). (C-F) mRNA expression levels of osteogenic markers: (C) ALP, (d) COL-1, (E) OPN, and (F) RUNX2 at days 4, 7, and 14. (n = 3, * P < . 05; ** P < . 01; *** P < . 001; **** P < . 0001, ns, no significance, data are described as the mean ± SD, comparisons were analyzed by two-way ANOVA adjusted by the Tukey test). Scale bars in (a) = 200 μm.

    Article Snippet: Analogously, osteogenic differentiation was induced in 10% FBS α-MEM medium supplemented with osteogenic induction reagents (consisting of 0.1 μM/L dexamethasone, 10 mM/L β-glycerophosphate, and 50 μg/mL ascorbic acid; MP Biomedicals and Sigma, US) for 21 days.

    Techniques: Staining, Software, Expressing